%0 Journal Article
%T Cloning and Characterization of a Novel S-Adenosyl-L-Methionine Synthase Gene in Tobacco<br>烟草中一条新的S-腺苷甲硫氨酸合成酶基因的克隆及表达分析
%A YU Tao
%A ZHI Li-Feng
%A PENG Lun
%A LI Yang-Sheng
%A ZHU Ying-Guo y
%A College of Life Sciences
%A Wuhan University
%A Wuhan
%A China
%A <br>余涛
%A 支立峰
%A 彭论
%A 李阳生
%A 朱英国
%J 植物科学学报
%D 2004
%I 
%X Differential display method was used to identify novel genes regulated by the ethylene generator,ethephon in tobacco plants.A full length cDNA of S-Adenosyl-L-Methionine synthase (SAMS2) which contains 1350 bp was isolated by this way combined with RACE technology.Sequence analysis revealed that this gene shared 90% similarity in gene sequence and 96% similarity in protein sequence with a known SAMS (SAMS1) gene though they have low homologous in the 5' and 3' uncoden region.RT-PCR analysis indicated that the expression of SAMS1 was induced by the treatment of high temperature but has no response to oxidative stress induced by methyl viologen (MV) and NaCl stress,while SAMS2 was up-regulated by all the three stress treatment.
%K S-Adenosyl-L-Methionine synthase
%K S-Adenosyl-L-Methionine
%K Ethephon
%K Stress<br>S-腺苷甲硫氨酸合成酶
%K S-腺苷甲硫氨酸
%K 乙烯利
%K 逆境胁迫
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=14A7BB24085D4806&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=E158A972A605785F&sid=CAA7BAE04CB631A1&eid=E2B9962CCD971A0D&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=8&reference_num=25