%0 Journal Article
%T Studies on the Cryopreservation of Suspension Culture Cell in Glycyrrhiza inflata Batal by Vitrification Approach
甘草悬浮细胞的玻璃化法超低温保存研究
%A YU Lu
%A YANG Ying
%A LI Shuo
%A FU Chun-Hua
%A YU Long-Jiang
%A
余露
%A 杨英
%A 李硕
%A 付春华
%A 余龙江
%J 植物科学学报
%D 2007
%I
%X A procedure had been developed for cryopreservation of Glycyrrhiza inflata Batal suspension cultures by vitrification.High flavonoid-yield G.inflata Batal suspension cultures are valuable materials but hard to be preserved.The key point of this research is how to reduce the damage of cell caused by the sharp change of pressure.So setting up a suitable method must depend on various important factors such as the duration of regrowth,the harvest time,the duration of dehydration,the sucrose concentration of washing solution,etc.Survival rate of G.inflata Batal suspension culture cell was 82.9% by TTC examination,and regeneration rate reached to 80.4%.
%K Liquorice(Glycyrrhiza inflata Batal)
%K Suspension culture cell
%K Vitrification
%K Cryopreservation
甘草
%K 悬浮细胞
%K 玻璃化
%K 超低温保存
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=91711145D962C26AA87748441C88E30B&yid=A732AF04DDA03BB3&vid=C5154311167311FE&iid=94C357A881DFC066&sid=8C5DE51F0A009A0F&eid=4D4C81DBA842B7BD&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=0&reference_num=18