%0 Journal Article
%T RAPD Analysis and Construction of DNA Fingerprints in Five Flos lonicerae Lines<br>金银花五个品系的RAPD分析及DNA指纹图谱的建立
%A YANG Fei
%A ZHANG Min
%A PENG Xing-Yang
%A WEI Jian-Jun
%A LI Li-Jia
%A <br>杨飞
%A 张敏
%A 彭兴扬
%A 魏建军
%A 李立家
%J 植物科学学报
%D 2007
%I 
%X RAPD technique has been used to construct DNA fingerprints and analyze genetic diversity in Flos lonicerae.From eighty primers,twenty-five primers were selected which can amplify clear and reproducible polymorphic bands.The PCR results showed that a total of 170 bands were amplified,of which 153 bands were polymorphic.Interestingly,primer SBSD06 generated distinct fingerprinting patterns that could distinguish each of the five lines.Genetic similarity correlation and the cluster analysis indicated the abundant genetic diversity in five F.lonicerae lines.A dendrogram was constructed based on genetic distances using UPGMA method.The dendogram reflected that five F.lonicerae lines clustered into two groups,and this was in accordance with the result of morphologic taxa.
%K Flos lonicerae
%K Molecular marker
%K Genetic diversity<br>金银花
%K 分子标记
%K 遗传多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=5D429288165D53EB&yid=A732AF04DDA03BB3&vid=C5154311167311FE&iid=38B194292C032A66&sid=6CCE24D86D03D083&eid=9CA95D22FC1D537C&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=0&reference_num=17