%0 Journal Article
%T Systemic PCD occurs in TMV-tomato interaction<br>
%A ZHOU ShuMin
%A LIU WenNa
%A KONG LinAn &
%A WANG Mao
%A <br>
%J 中国科学C辑(英文版)
%D 2008
%I Springer
%X In hypersensitive response (HR), programmed cell death (PCD) is reported as a powerful defense mechanism in plant immune responses to pathogen. However, little is known about the PCD in sys-temic acquired resistance (SAR). Using tobacco mosaic virus (TMV) to infect the tomato (Lycopersicon esculentum cv. Jiafen 16) we found that localized TMV-infection could induce cell death in the uninoculated parts of the tomatoes, where the enzyme-linked immunosorbent assay (ELISA) showed no spreading virus. The biological and molecular characterization of this cell death was shown as fol-lowing: chromatin condensed and formed peripheral conglomeration in nuclei; cell nucleus were TUNEL positive labeled; genomic DNA was fragmented and showed DNA laddering; mitochondria and chloroplast were disrupted; tonoplast and plasma membrane were shrunk and degradated. These re-sults suggested that with an absence of TMV spread, the local TMV-infection on certain tomato leaves could induce systemic PCD in the root-tips, stem-apices and uninoculated leaves. The systemic PCD has various initiation and synchronization in such tissues and is distinct in inducement and exhibition from HR-PCD and SAR.
%K programmed cell death
%K systemic PCD
%K TUNEL assay
%K DNA ladder
%K TMV
%K tomato<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=01DB46D80240F3B692F5EAED727F68D6&yid=67289AFF6305E306&vid=987EDA49D8A7A635&iid=708DD6B15D2464E8&sid=9F481C73BF82C48F&eid=CBC69BEA05C12902&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=49