%0 Journal Article
%T Studies on Factors in Cryopreservation of Taxus Callus<br>红豆杉愈伤组织超低温保存有关因素的研究
%A YE Fang
%A XIA Sheng
%A MEI Xing-Guo
%A <br>叶芳
%A 夏胜
%A 梅兴国
%J 植物科学学报
%D 2001
%I 
%X The experiment compares the main factors of cryopreservation of Taxus callus. The results show that with the differences of preculture time, saccharose content of culture medium, cryoprotectants and freezing method, the cell vitality varies remarkably. After Taxus callus was precultured in No.62 medium with 8% saccharose for 6 days, the TTC value of Taxus calluses after freezing and storage in LN(liquid nitrogen) was high. The most effective cryoprotectant is the mixture of 10% DMSO and 10% sorbital. The effectiveness of the step freezing and slow freezing methods was much better than that of the rapid freezing method.
%K Cryopreservation
%K Cell vitality
%K Taxus callus<br>超低温保存
%K 细胞活力
%K 红豆杉愈伤组织
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=28959F3B5C6C1BAD&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=E158A972A605785F&sid=170CE8B011EA4FD9&eid=717CC18E05F2AFA0&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=4&reference_num=9