%0 Journal Article
%T The Effects of Dark and Light on Growth and Physiological and Biochemical Characteristics in Cell Culture of Catharanthus roseus(L.)G.Don<br>黑暗和光照对长春花培养细胞生长和生理生化特性的影响
%A GUO Sheng-Juan
%A LIU Shu-Nan
%A FENG Ling-Ling
%A ZHOU Ji-Yuan
%A <br>郭胜娟
%A 刘树楠
%A 冯玲玲
%A 周吉源
%J 植物科学学报
%D 2004
%I 
%X Based on young leaves of Catharanthus roseus (L.) G. Don, callus is induced on the solid medium of MS+NAA 0.5 mg/L +2,4-D 0.5 mg/L +6 BA 2.0 mg/L. Two groups of callus are cultured in the dark and light, respectively. The influeces of light on the cell growth and physiological and biochemical characteristics are studied. The results show that: both the cell growth curves are like "S", and their growth circles are about 27 days. Activities of peroxides (POD) and superoxide dismutase (SOD) show two peaks. We also find that the alkaloid production increased during the growth circles and can get the most production at the 27th day.
%K Callus
%K Cell growth
%K Peroxides (POD)
%K Superoxide dismutase (SOD)
%K Alkaloid
%K Catharanthus roseus (L
%K ) G
%K Don<br>愈伤组织
%K 细胞生长
%K 过氧化物酶
%K 超氧化物歧化酶
%K 生物碱
%K 长春花
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=22B582870DDBCB29&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=0B39A22176CE99FB&sid=58F693790F887B3B&eid=12DC19455C3A2FA8&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=5&reference_num=13