%0 Journal Article
%T Prevention of pathological change and cognitive degeneration of Tg2576 mice by inoculating Aβ1–15 vaccine<br>
%A HU JinJia
%A LI GuoYing
%A WANG HuaQiao
%A LIN Xian &
%A YAO ZhiBin
%A <br>
%J 中国科学C辑(英文版)
%D 2008
%I Springer
%X This study aims to discuss the effect of preventing pathological changes and cognitive degeneration of Tg2576 mice by inoculating the subunit fragment of Abeta vaccine. Thirty-two Tg2576 mice were randomly divided into four groups, each having eight mice: Group I, the control group, inoculated with adjuvants; Group II, the Abeta(42) group, inoculated with Abeta(42) vaccine; Group III, the Abeta(1-15) group, inoculated with Abeta(1-15) vaccine; and Group IV, the Abeta(36-42) group, inoculated with Abeta(36-42) vaccine. The titer of the serum antibody against Abeta(42) (Group II) was significantly higher than that of the control group (Group I), and a low level of antibodies could be detected in the brain homogenate in the three vaccine-inoculated groups. Morris water maze test showed that the Abeta(42) group, Abeta(1-15) group and Abeta(36-42) group were obviously improved compared with the control group. The cultured splenocytes sampled from each group were induced by Con A or their respective antigens, and the cell proliferation of the three vaccine-inoculated groups was significantly higher than that of the control group. In the Abeta(42) group, IL2 and IFN-gamma were relatively low and IL4 and IL10 were relatively high. By contrast, IL4 and IL10 were much higher in the Abeta(1-15) group and IL2 and IFN-gamma were much higher in the Abeta(36-42) group. The immunohistochemical test showed a large number of senile plaques in the brain cortex and hippocampus of the mice in the control group, no senile plaque in the brain of the Abeta(1-15) group and Abeta(42) group mice, and a small number of senile plaques in the brain of the Abeta(36-42) group mice. The results suggest that the subunit fragment of Abeta(1-15) vaccine could prevent not only cognitive and behavioral degeneration but also Abeta deposition and formation of senile plaques in Tg2576 mice.
%K Alzheimer’
%K s disease
%K β
%K -amyloid peptide
%K vaccine
%K Tg2576 mice
%K senile plaque<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=A1AF32850A43C03891F0D897A7D2194C&yid=67289AFF6305E306&vid=987EDA49D8A7A635&iid=5D311CA918CA9A03&sid=6EDA906E07280FB0&eid=626A0FE8E3130AB5&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=19