%0 Journal Article
%T Extraction and RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile (CMS) Line in Broccoli<br>青花菜细胞质雄性不育系线粒体DNA的提取与RAPD分析
%A ZHU Yu-Ying
%A YANG Xiao-Feng
%A HOU Rui-Xian
%A LI Shi-Jun
%A WU Qin-Sheng
%A <br>朱玉英
%A 杨晓锋
%A 侯瑞贤
%A 李式军
%A 吴琴生
%J 植物科学学报
%D 2006
%I 
%X DNA from the mitochondrion(mtDNA) of CMS line BC7-19 and its maintaner of broccoli(Brassica oleracea L.var.italica Plenck.) was extracted by molecular bio-technique,and was marked(using) RAPD reaction.The result showed that the purity of the extracted mtDNA was high by sepharose(electrophoresis).And the result also showed that a special fragment,BMS_(1000),with about(1000) bp amplificated by primer S66,was peculiar for the mtDNA of male sterile line by RAPD reaction of 40 primers,and the special fragment was initially considered to be relevant to male sterility after sequence analysis.
%K Broccoli(Brassica oleracea L
%K var
%K italica Plenck
%K )
%K Male sterile line
%K mtDNA
%K RAPD<br>青花菜
%K 雄性不育系
%K 线粒体DNA
%K RAPD
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=1B41C0FE00783241&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=B31275AF3241DB2D&sid=42D7028D961473F8&eid=37F781FD8E744761&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=3&reference_num=7