%0 Journal Article
%T Construction and Characterization of A Recombinant Fowlpox Virus Containing HIV-1 Multi-epitope-p24 Chimeric Gene on Mice<br>Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice
%A Zhang LiShu Jin NingYi Song YingJin Wang Hong Ma HeWen Li ZiJian&
%A Jiang WenZheng
%A <br>
%J 中国科学C辑(英文版)
%D 2007
%I Springer
%X The epidemic of HIV/AIDS is sweeping across the world. It is of great importance to figure out new ways to curb this disease. Epitope-based vaccine is one of these solutions. In this study, a chimeric gene was obtained by combination of a designed HIV-1 multi-epitope gene (MEG) and HIV-1 p24 gene. A recombinant plasmid pUTA2-MEGp24 was then constructed by inserting MEGp24 gene into the downstream of the promoter (ATI-P7.5×20) of fowlpox virus (FPV) transfer vector pUTA2. The recombinant plasmid and wild-type FPV 282E4 strain were then co-transfected into CEF cells and homologous recombination occurred. A recombinant virus expressing HIV-1 protein MEGp24 was screened by genome PCR and Western blot assay. Large scale preparation and purification of the recombinant fowlpox virus (rFPV) were then carried out. BALB/c mice were immunized intramuscularly with the rFPV for three times on day 0, 14 and 42. Mice were executed and sampled one week after the third inoculation. Anti-HIV-1 antibody in serum and Th1 cytokines in the supernatant of cultured spleen cells were assayed by ELISA. The count of T lymphocyte subsets and the CTL activity of spleen lymphocytes were analyzed by flow cytometry and lactate dehydrogenase (LDH) release assay, respectively. The results showed that HIV-1 specific antibody in serum and increased T lymphocyte subsets (CD4+ T, CD8+ T) were detected in the immunization group. CTL target-killing activity and higher secretion of Th1 cytokines (IFN-γ and IL-2) of spleen lymphocytes stimulated by H-2d-restricted CTL peptide were observed in immunized mice. We concluded that the rFPV may induce HIV-1 specific immunity especially cellular immunity in mice. Supported by the China “863” Program (Grant No. 2003AA219051)
%K HIV-1
%K multi-epitope
%K recombinant
%K fowlpox virus
%K immune response<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=D943E7F1D898C93068C896EB64FF2216&yid=A732AF04DDA03BB3&vid=771152D1ADC1C0EB&iid=0B39A22176CE99FB&sid=D2742EEE6F4DF8FE&eid=1D67BE204FBF4800&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=1&reference_num=27