%0 Journal Article
%T Preparation of Total DNA from Desert Plant Calligonum L.<br>沙拐枣属（Calligonum L.）7种植物总DNA提取方法的改进及鉴定
%A JIN Ling
%A LI Ming
%A ZHANG Yong
%A BAI Lei
%A AN Li-zhe
%A CHEN Tuo
%A <br>晋 玲
%A 李 鸣
%A 张 勇
%A 白 蕾
%A 安黎哲
%A 陈 拓
%J 中国沙漠
%D 2007
%I 
%X We introduced a better method which do best for the purity and concentration of total DNA of seven Calligonum species: Calligonum mongolicum Turcz.,C.junceum(Fisch.et Mey.) Endl.,C.aphyllum(Pall.) G rke,C.rubicundum Bge.,C.cordatum E.Kor.ex N.Pavl.,C.densum Borszcz.,C.arborescens Litv..This method is an improved one upon material and method based on the traditional CTAB method.The result showed that it could effectively eliminate the affection of secondary materials to extracted total DNA from Calligonum,and is suitable for PCR of ISSR and other genetic research.Also,it provides a reliable experimental method for relevant researches on Calligonum.
%K ISSR<br>沙拐枣属
%K 总DNA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=869B153A4C6B5B85&jid=F62EDFC6E63C5D025C6AE9D0171B7C05&aid=AB17A82749A16FB6&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=38B194292C032A66&sid=4D4C81DBA842B7BD&eid=3FC4D669D19FF0C6&journal_id=1000-694X&journal_name=中国沙漠&referenced_num=0&reference_num=13