%0 Journal Article
%T Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosomes deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1
%A LI Feng
%A LI Ying JIANG Wei WANG Zhenfang LI Jilun
%A <br>LI Feng
%A LI Ying
%A JIANG Wei
%A WANG Zhenfang & LI Jilun . State Key Laboratory for Agro-biotechnology and
%J 中国科学C辑(英文版)
%D 2005
%I Springer
%X A magnetosome deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1 was generated by mini-Tn5 transposon mutagenesis, and a 5045-bp fragment flanking mini-Tn5 in NM4 was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved six putative open reading frames (ORFs); the mini-Tn5 was inserted into ORF4. Functional complementary test indicated that the 5045-bp fragment was required for biosynthesis of mag-netosomes in M. gryphiswaldense MSR-1. The protein encoded by ORF4 had 25% of identity with the chemotaxis protein CheYIII of Caulobacter crescentus CB15, and the protein encoded by ORF4 contained a conserved signal receiver domain that can receive the signal from the sensor partner of the bacterial two-component systems. It was suggested that the protein en-coded by ORF4 may take part in the signal transduction relating to biosynthesis of magneto-somes.
%K Magnetospirillum gryphiswaldense
%K magnetosome deleted mutant
%K gene cloning
%K functional analysis<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=20AE8CFB1D4ABE36FBA06999CC7A404A&yid=2DD7160C83D0ACED&vid=B6DA1AC076E37400&iid=B31275AF3241DB2D&sid=F6467E7C54ED952D&eid=E04FC1B5BC47587B&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=28