%0 Journal Article
%T Chondrogenic differentiation of mouse embryonic stem cells promoted by mature chondrocytes<br>
%A XIE Feng
%A ZHANG WenJie
%A CHEN FanFan
%A ZHOU GuangDong
%A CUI Lei
%A LIU Wei &
%A CAO YiLin
%A <br>
%J 中国科学C辑(英文版)
%D 2008
%I Springer
%X In order to direct embryonic stem (ES) cells to differentiate into chondrocytes, a chondrogenic envi-ronment provided by mature chondrocytes was investigated. Flk-1 positive cells sorted from pre-differentiated mouse ES cells were mixed with adult porcine articular chondrocytes, seeded on biodegradable scaffolds, and then implanted subcutaneously into nude mice. The cell-scaffold com-plexes formed cartilage tissues after 4 weeks, which was demonstrated by histology and anti-type II collagen antibody staining. Positive staining of mouse Major Histocompatibility Complex class I molecules confirmed that part of the chondrocytes were derived from mouse ES cells. The current study established a new approach for directing ES cell differentiation.
%K chondrocytes
%K co-culture
%K differentiation
%K embryonic stem cells<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=0BCF3446E8FDB29560D63189AC4E7615&yid=67289AFF6305E306&vid=987EDA49D8A7A635&iid=9CF7A0430CBB2DFD&sid=786A9BAE5CF9B9EE&eid=0358FC3DBCE8E14D&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=8