%0 Journal Article
%T Involvement of insulin in early development of mouse one-cell stage embryos<br>
%A YU BingZhi
%A ZHANG Zhe
%A DENG Xin
%A XU XiaoYan
%A FENG Chen
%A LI YanXiao
%A CUI Cheng
%A SU WenHui
%A ZHAO HongMei &
%A YU DaHai
%A <br>
%J 中国科学C辑(英文版)
%D 2008
%I Springer
%X Recent studies have suggested that growth factors and hormones play important roles in cell prolif-eration and differentiation during early embryonic development. In the present study, we examined the expression and localization of insulin in the mouse oocytes and one-cell stage embryos by quantitative ELISA, RT-PCR, Western blot and immunofluorescence. In the mouse oocytes and one-cell stage em-bryos, expression of insulin was uniformly distributed in the cytoplasm. We also examined the expres-sion, activity and localization of mTOR (mammalian target of rapamycin) and p70S6K. The expression of mTOR and p70S6K was not significantly different at the cell cycle of mouse one-cell stage embryos. mTOR and S6K were distributed evenly in the cytoplasm at G1, G2 and M phase phase, but at S phase, the distribution of mTOR and S6K was around the pronucleus. At different phases, the activity of mTOR fluctuated. We also used the PI3K specific inhibitor-Wortmannin to investigate the cleavage rate of eggs. The result showed that the rate obviously decreased. When the mTOR specific inhibitor Rapa-mycin was used, the first mitotic division of the mouse one-cell stage embryo was delayed. These re-sults suggested that insulin was expressed both in mouse oocytes and one-cell stage embryos, and may play functional roles in regulation of mouse early embryogenesis by activating the signal pathway of PI3K/PKB/mTOR/S6K.
%K mouse
%K embryo
%K insulin
%K mTOR
%K S6K<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=87581C510C57FDCF3AFCC12EE484DAA2&yid=67289AFF6305E306&vid=987EDA49D8A7A635&iid=9CF7A0430CBB2DFD&sid=70081086C74F4110&eid=1F7317C17A9AF4FA&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=24