%0 Journal Article
%T Growth phase-dependent expression of proteins with decreased plant-specific N-glycans and immunogenicity in tobacco BY2 cells<br>
%A BoJiao Yin
%A NuoYan Zheng
%A Yin Li
%A SanYuan Tang
%A LiMing Liang
%A Qi Xie
%A <br>
%J 中国科学C辑(英文版)
%D 2009
%I Springer
%X Plants possess some desirable characteristics to synthesize recombinant glycoproteins for pharma-ceutical application. However, the mammalian glycoproteins produced in plants are somewhat different from their natural counterparts in terms of N-glycoforms. The immunogenicity of plant-specific glyco-epitopes is the major concern in human therapy. Here, the distribution of N-glycans in different growth phases of tobacco BY2 cells and their immunogenicity in mice were determined. It was ob-served that the percentage of β1,2-xylose and α1,3-fucose in proteins of growing cells increased and the corresponding protein extracts caused accelerated immune response in mice. Based on this ob-servation, the recombinant erythropoietin in BY2 cells was expressed and characterized, and Western blot analysis showed that the recombinant erythropoietin contained a relatively small amount of plant-specific glyco-epitopes in the early phase of culture growth. This study may provide a simple but effective strategy for the production of therapeutic glycoproteins with human-like N-glycan structures in plant hosts to avoid a great allergenic risk.
%K BY2 cell
%K N-glycans
%K protein expression
%K immunogenicity<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=5720713FB3DCB25CD47F7D3678E2D32E&yid=DE12191FBD62783C&vid=286FB2D22CF8D013&iid=5D311CA918CA9A03&sid=547991B346A6585E&eid=4C49EFA0A53C9DF0&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=29