%0 Journal Article
%T Primary functional identification of gene TMSG-1
%A MA Chunshu
%A NING Junyu
%A YOU Jiangfeng
%A LIU Lin
%A WANG Jieliang
%A CUI Xianglin
%A WU Bingquan
%A ZHENG Jie
%A <br>马春树
%A 宁钧宇
%A 由江峰
%A 柳林
%A 王洁良
%A 崔湘琳
%A 吴秉铨
%A 郑杰
%J 中国科学C辑(英文版)
%D 2003
%I Springer
%X TMSG-1 was a tumor metastasis-related gene identified using mRNA differential dis-play, whose expression level was lower in cancer cell lines with higher metastatic potential and in tumor tissue with metastasis. TMSG-1 was transfected to prostate cancer cell line (PC-3M-1E8) with high metastatic potential to observe the effects of increased expression of TMSG-1 on V-ATPase activity, intracellular pH and cell apoptosis. Subcellular localization of the encoded pro-tein of TMSG-1 was determined by using GFP. Results showed that there were no differences of V-ATPase activity among parental PC-3M-1E8 cell line, pcDNA3 transfectant and anti-TMSG-1 transfectant, whereas the V-ATPase activity was significantly higher in TMSG-1 transfectant than that in parental PC-3M-1E8 cell line, pcDNA3 transfectant and Anti-TMSG-1 transfectant (p<0.001). Intracellular pH (pHi) was detected by using the pH-dependent fluorescence probe BECEF. Re-sults showed the pHi was significantly increased in TMSG-1 transfectant. Cell apoptosis assay demonstrated cell apoptosis was significantly higher in -1 transfectant (p<0.01) and BCL2 expres-sion was down regulated. Subcellular localization of TMSG-1 protein showed TMSG-1 was a transmembrane protein, which predicted TMSG-1 protein was located in cytoplasm system, such as endoplasmic reticulum and mitochondrial. These results indicated TMSG-1 up regulation in prostate cancer cell line could promote V-ATPase activity, increase pHi and cell apoptosis, and inhibit the expression of BCL2.
%K prostate cancer
%K gene transfection
%K pH
%K apoptosis<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=A9A95F8F54C94DC624D2CB01792FFF7B&yid=D43C4A19B2EE3C0A&vid=D997634CFE9B6321&iid=B31275AF3241DB2D&sid=9A596D09E9486F3E&eid=7C13E30F5EDBE7AB&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=0