%0 Journal Article
%T Enhanced production of lutein in heterotrophic Chlorella protothecoides by oxidative stress<br>
%A Dong Wei
%A Feng Chen
%A Gu Chen
%A XueWu Zhang
%A LongJun Liu
%A Hao Zhang
%A <br>
%J 中国科学C辑(英文版)
%D 2008
%I Springer
%X The fast growing unicellular green microalgae Chlorella protothecoides has attracted interest as a promising organism for commercial production of a high-value carotenoid, lutein, by heterotrophic fermentation. Effects of two oxidant-forming reactive oxygen species (ROS) on the biomass concentration, and yield and content of lutein in batch culture of heterotrophic Chlorella protothecoides were investigated in this study. The addition of 0.1 mmol/L H2O2 and 0.01 mmol/L NaClO plus 0.5 mmol/L Fe2+ to the culture led to the generation of ·OH and enhanced the lutein content from 1.75 to 1.90 and 1.95 mg/g, respectively. The lutein content further increased to 1.98 mg/g when 0.01 mmol/L H2O2 and 0.5 mmol/L NaClO were added to generate 1O2. The maximum yield of lutein (28.5, 29.8 and 31.4 mg/L) and a high biomass concentration (15.0, 15.3 and 15.9 g/L) were also achieved through the above treatments. The results indicated that 1O2 could promote lutein formation and enhance lutein production in heterotrophic Chlorella protothecoides. Moreover, 1O2 produced from the reaction of H2O2 and NaClO was more effective in enhancing lutein production and reducing biomass loss than ·OH from the reaction of H2O2 or NaClO plus Fe2+. Supported by the National Key Project of Sci & Tech Supporting Programs Funded by Ministry of Science & Technology of China (Grant No. 2006BAD27B03), Sci & Tech Project of Guangzhou (Grant No. 2005Z3-E0331) and Sci & Tech Project of Guangdong (Grant No. 20052050166)
%K Chlorella protothecoides
%K heterotrophic
%K lutein
%K oxidative stress
%K ROS<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=2577C7CE568296C570339A658A1CEE14&yid=67289AFF6305E306&vid=987EDA49D8A7A635&iid=59906B3B2830C2C5&sid=61EDB4BBA42E40FF&eid=8DEAC935CD342902&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=35