%0 Journal Article
%T Mechanism of enhancement of prochymosin renaturation by solubilization of inclusion bodies at alkaline pH
%A ZHANG Zhizhou
%A ZHANG Yuying
%A YANG Kaiyu
%A <br>张治洲
%A 张渝英
%A 杨开宇
%J 中国科学C辑(英文版)
%D 1997
%I Springer
%X The renaturation efficiency of recombinant prochymosin depends on not only the renaturation condi-tions but also the solubilization (denaturation) conditions. Compared with pH 8, solubilization of prochymosin-contain-ing inclusion bodies at pH 11 (8 mol/L urea) results in onefold increase of renaturation efficiency ( ~ 40% vs. ~ 20 % ). Alkaline pH facilitates the solubilization of inclusion bodies via the breakage of intermolecular disulfide bonds. Moreover, alkaline pH renders prochymosin molecules to be in a more reduced and more unfolded state which undergoes refolding readily.
%K solubilization of inclusion bodies
%K renatnration of recombinant prochymosin<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=601A6814A6A25D8EC09F2F5631A58A9E&yid=5370399DC954B911&vid=1371F55DA51B6E64&iid=0B39A22176CE99FB&sid=954CE65414DD94CA&eid=A58CF3BAE79427D0&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=10