%0 Journal Article
%T Isolation of Egg Cells from Allium fistulosum<br>葱卵细胞的分离
%A Mingxia Jian
%A Yanan Zhang
%A Yaying Wang
%A Huiqiao Tian
%A <br>菅明霞
%A 张亚楠
%A 王雅英
%A 田惠桥
%J 植物学报
%D 2009
%I 
%X Viable egg cells of Allium fistulosum were isolated by enzymatic digestion and mechanical dissection. The ovules were incu-bated in an enzymatic solution containing 0-0.8% pectolyse Y-23, 0-1.2% pectinase (Serva), 0-1.2% cellulase (Onozuka R-S), 0-1% hemicellulase (Sigma) and 6%-12% mannitol for 20-40 min to remove the outer integument. Then ovules were tr-ansferred into an isolating solution without enzymes for mechanical dissection. The ovules were cut from the middle part, and the micropylar part was gently extruded by use of a dissection needle. In general, the three cells of the egg apparatus spilled out from the cut end of the ovule. The enzymatic solution containing 0.2% pectolyse Y-23, 0.8% pectinase (Serva), 0.8% cellulase (Onozuka RS), 0.5%hemicellulase (Sigma) and 10% mannitol was best for isolating about 18 egg cells from 30 ovules in 2 h. The isolated egg cells could be used for in vitro fertilization to explore the fertilization mechanism and for egg development with molecular biological methods.
%K <br>葱
%K 卵细胞
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=8CF3C184602FEE7DB3A83B8D02764E35&yid=DE12191FBD62783C&vid=1AE5323881A5ECDC&iid=38B194292C032A66&sid=3622B70F9C54A9CC&eid=406BF8ED3BCE1927&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=17