%0 Journal Article
%T Molecular cloning of GA-suppressed G2 pea genes by cDNA RDA
%A ZHU Yuxian
%A ZHANG Yifeng
%A LI Huiying
%A <br>朱玉贤
%A 张翼凤
%A 李慧英
%J 中国科学C辑(英文版)
%D 1997
%I Springer
%X GA-treated and non-treated G2 pea cDNAs were compared using a newly developed method called cDNA representational difference analysis (cDNA-RDA), and several GA-suppressed mRNAs were found. After cloning of the larger fragments PGAS1-3 ( pea GA-suppressed cDNA 1-3), they were demonstrated to be expressed only in pea tissue not treated with GA3 through Northern analysis. Compared with subtractive hybridization and differ-ential display techniques, this method not only can be easily manipulated but also has a relatively low rate of false posi-tive and is highly repetitive. It is the major progress in molecular cloning techniques.
%K G2 pea
%K RDA
%K molecular cloning
%K GA-suppressed genes<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=BDB978E3C9A74657A8DAF886DA1C6E4A&yid=5370399DC954B911&vid=1371F55DA51B6E64&iid=E158A972A605785F&sid=9EF602EA28138BEA&eid=663FF78B2ADE7A2D&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=5&reference_num=15