%0 Journal Article
%T Construction of a novel kind of expression plasmid by ho-mologous recombination in Saccharomyces cerevisiae<br>Construction of a novel kind of expression plasmid by homologous recombination in Saccharomyces cerevisiae
%A CHEN Xiangling
%A YUAN Hanying
%A HE Wei
%A HU Xianghua
%A LU Hong
%A LI Yuyang
%A <br>CHEN Xiangling
%A YUAN Hanying
%A HE Wei
%A HU Xianghua
%A LU Hong & LI Yuyang State Key Laboratory of Genetic Engineering
%A School of Life Sciences
%A Fudan University
%A Shanghai
%A China
%J 中国科学C辑(英文版)
%D 2005
%I Springer
%X Based on a previously used plasmid pHC11, a new plasmid pHC11R was constructed. Cutting plasmid pHC11R with proper restriction enzymes, the resulting larger DNA fragment pHC11R' was co-transformed with a PCR amplified expression cassette of human IFNalpha2b into yeast. By means of the homologous sequences at both ends of two DNA fragments, a novel expression plasmid pHC11R-IFNalpha2b was formed via homologous recombination in the yeast. Compared with pHC11-IFNalpha2b, the expression plasmid pHC11R-IFNalpha2b was smaller in size and in absence of antibiotic resistant gene. The stability and copy number of pHC11R-IFNalpha2b were greatly increased and the expression level of heterologous protein was improved. As the derivatives of pHC11R, a series of recombination expression vectors pHRs containing different combination of expression elements were developed. This led to a rapid and powerful method for cloning and expressing of different genes in yeast.
%K homologous recombination
%K Saccharomyces cerevisiae
%K expression plasmid<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=0178E0428C53D4479CB65355ABABA9E8&yid=2DD7160C83D0ACED&vid=B6DA1AC076E37400&iid=E158A972A605785F&sid=6C069F4B248105A5&eid=50EA2A80A7D254EF&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=27