%0 Journal Article
%T The transfection of embryonic stem cells with Tet-on system and its responsiveness to doxycycline
%A WANG Yan
%A CONG Xiaoqian
%A LIU Deli
%A ZHANG Wenjie
%A CUI Lei
%A LIU Wei
%A CAO Yilin
%A <br>WANG Yan
%A CONG Xiaoqian
%A LIU Deli
%A ZHANG Wenjie
%A CUI Lei
%A LIU Wei & CAO Yilin
%J 中国科学C辑(英文版)
%D 2005
%I Springer
%X We transiently transfected pTet-on and pTRE2hyg-luciferase into the mouse em-bryonic stem cells (ESCs) using lipofectamine, and analyzed its inductive effect by adding serial concentrations of doxycycline (DOX). The results showed that in the transfected group, the luciferase activity of the cells was gradually increased along with the increasing concentration of DOX. While in the non-transfected group, the luciferase activity was not detectable even with DOX treatment. This indicated that the ESCs transfected with Tet-on system could response to DOX very well, and the regulation of target gene expression is dose dependent.
%K gene expression
%K embryonic stem cell<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=A28E0299E2AFA7812475EF79F01C7322&yid=2DD7160C83D0ACED&vid=B6DA1AC076E37400&iid=E158A972A605785F&sid=F416A9924F23B020&eid=85002451B65CE0D1&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=14