%0 Journal Article
%T Ethanol disrupts the formation of hypochord and dorsal aorta during the development of embryonic zebrafish
%A Qian Linxi
%A Wang Yuexiang
%A Jiang Qiu
%A Zhong Tao
%A Song Houyan
%A <br>QIAN Linxi
%A WANG Yuexiang
%A JIANG Qiu
%A ZHONG Tao & SONG Houyan Key Laboratory of Molecular Medicine
%A Ministry of Education
%A Shanghai Medical School of Fudan University
%A Shanghai
%A China
%J 中国科学C辑(英文版)
%D 2005
%I Springer
%X Exposure to ethanol during human embryonic period has severe teratogenic effects on the cardiovascular system. In our study, we demonstrated that ethanol of gradient concentra-tions can interfere with the establishment of circulatory system in embryonic zebrafish. The ef-fective concentration to cause 50% malformations (EC50) was 182.5 mmol/L. The ethanol pulse exposure experiment displayed that dome stage during embryogenesis is the sensitive time window to ethanol. It is found that 400 mmol/L ethanol pulse exposure can induce circulatory defects in 43% treated embryos. We ruled out the possibility that ethanol can interfere with the process of hematopoiesis in zebrafish. By employing in situ hybridization with endothelial bio-marker (Flk-1), we revealed that ethanol disrupts the establishment of trunk axial vasculature, but has no effect on cranial vessels. Combined with the results of semi-thin histological sections, the in situ hybridization experiments with arterial and venous biomarkers (ephrinB2, ephB4) sug-gested that ethanol mainly interrupts the development of dorsal aorta while has little effect on axial vein. Further study indicated the negative influence of ethanol on the development of hy-pochord in zebrafish. The consequent lack of vasculogenic factors including Radar and Ang-1 partly explains the defects in formation and integrity of dorsal aorta. These results provide im-portant clues to the study of adverse effects of ethanol on the cardiovascular development in human fetus.
%K Ethanol
%K zebrafish
%K dorsal aorta
%K hypochord
%K vasculogenesis<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=058824BFD005001456476F6585D6DC1C&yid=2DD7160C83D0ACED&vid=B6DA1AC076E37400&iid=B31275AF3241DB2D&sid=C7B13290323C226E&eid=D9D6C3CD78BED2C5&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=2&reference_num=21