%0 Journal Article
%T Research of blastocyte-like structure in chicken
%A Li Jia Pan Qiuzhen Li Junying Han Hongbing Sun Shufeng Yang Jun Xu Shuguang Tian Liang Lian Zhengxing
%A Yang Ning
%A Li Ning
%A <br>LI Jia
%A PAN Qiuzhen
%A LI Junying
%A HAN Hongbing
%A SUN Shufeng
%A YANG Jun
%A XU Shuguang
%A TIAN Liang
%A LIAN Zhengxing
%A YANG Ning
%A & LI Ning
%A . College of Animal Science and Technology
%A China Agricultural University
%A Beijing
%A China
%A . Laboratory of Animal Genetics and Breeding
%A Ministry of Agriculture
%A Beijing
%A China
%A . State Key Laboratory of Agrobiotechnology
%A Beijing
%A China
%J 中国科学C辑(英文版)
%D 2005
%I Springer
%X The chicken embryo is a classic model used to investigate embryonic development, gene expression, and tissue differentiation, and is also an important research tool in studying the animal functional genomics. The whole blastoderms of fresh unincubated eggs from White Leghorn chickens were collected with a paper ring, mechanically broken into small pieces and cultured in medium. Then the small pieces would develop into blastocyte-like structures (BLS), which could be facilitated by an addition of fetal bovine serum (FBS) to the primary culture and their diameter was nearly doubled from 12 to 24 h. The additional yolk had no positive effect on the development in the first 12 h but encouraged the BLSs attaching and inner cells differentiating instead in 24 h. The inner cells of the BLS showing a high alkaline-phosphatase activity similar to those in mouse embryonic stem (ES) cells and also expressing a large amount of the specific stage embryonic antigen-1 (SSEA-1) on the surface, which was known to be the characteristic of non-differentiated mouse and avian ES cells, could finally differentiate into nerve-like cells, fibroblast cells and so on in the medium. Leukemia inhibitory factor (LIF) facilitated the cells' proliferation and prevented differentiation in the suspended culture of the BLSs. So we drew the conclusion that the BLS obtained from broken blastoderm can be used to amplify avian ES cells so as to initiate a new method of producing transgenic chickens.
%K BLS
%K blastocyst-like structure
%K embryonic stem cells
%K ES cells
%K chicken<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=435624554C6591793FDBF489F7106EA2&yid=2DD7160C83D0ACED&vid=B6DA1AC076E37400&iid=94C357A881DFC066&sid=283B38DAD0D068F3&eid=8C8D895E58E44DBB&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=1&reference_num=13