%0 Journal Article
%T Cloned calves produced by nuclear transfer from cultured cumulus cells<br>Cloned calves produced by nuclear transfer from cultured cumulus cell
%A AN Xiaorong
%A GOU Kemian
%A ZHU Shien
%A GUAN Hong
%A HOU Jian
%A LIN Aixing
%A ZENG Shenming
%A TIAN Jianhui
%A CHEN Yongfu
%A <br>安晓荣
%A 苟克勉
%A 朱士恩
%A 关宏
%A 侯健
%A 林爱星
%A 曾申明
%A 田见辉
%A 陈永福
%J 中国科学C辑(英文版)
%D 2002
%I Springer
%X Short-term cultured cumulus cell lines (1-5BCC) derived from 5 individual cows were used in nuclear transfer (NT) and 1188 enucleated bovine oocytes matured in vitro were used as nuclear recipients. A total of 931 (78.4%) cloned embryos were reconstructed, of which 763 (82%) cleaved, 627 (67.3%) developed to 8-cell stage, and 275 (29.5%) reached blastocyst stage. The average cell number of blastocysts was 124+/-24.5 (n=20). In this study, the effects of donor cell sources, serum starvation of donor cells, time interval from fusion to activation (IFA) were also tested on cloning efficiency. These results showed that blastocyst rates of embryos reconstructed from 5 different individuals cells were significantly different among them (14.1%, 45.2%, 27.3%, 34.3%, vs 1.5%, P<0.05); that serum starvation of donor cells had no effect on blastocyst development rate of NT embryos (47.1% vs 44.4%, P>0.05); and that blastocyst rate (20.3%) of the group with fusion/activation interval of 2-3 h, was significantly lower than that of the 3-6 h groups (31.0%), while not significantly different among 3-4 h (P < 0.05), 4-5 h, and 5-6 h groups (P>/=0.05). Sixty-three thawed NT blastocysts were transferred to 31 recipient cows, of which 4 pregnancies were established and two cloned calves were given birth. These results indicate that serum starvation of cumulus cells is not a key factor for successful bovine cloning, while IFA treatment and sources of donor cells have effects on cloning efficiency.
%K cumulus cell
%K nuclear transfer
%K embryo freezing
%K cloned calf<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=8B87B07D1FFCB3C4D14946D063A6F1C0&yid=C3ACC247184A22C1&vid=94E7F66E6C42FA23&iid=0B39A22176CE99FB&sid=BC084ACE66B62CC8&eid=AA27B676BFCAA4BE&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=3&reference_num=20