%0 Journal Article
%T The effects of detergents DDM and beta-OG on the singlet excited state lifetime of the chlorophyll a in cytochrome b6f complex from spinach chloroplasts.<br>The effects of detergents DDM and β-OG on the singlet excited state lifetime of the chlorophyll a in cytochrome b6f complex from spinach chloroplasts
%A CHEN XiaoBo
%A ZHAO XiaoHui
%A ZHANG JianPing
%A LI LiangBi &
%A KUANG TingYun
%A <br>
%J 中国科学C辑(英文版)
%D 2007
%I Springer
%X The singlet excited state lifetime of the chlorophyll a (Chl a) in cytochrome b 6 f (Cyt b 6 f) complex was reported to be shorter than that of free Chl a in methanol, but the value was different for Cyt b 6 f complexes from different sources (～200 and ～600 ps are the two measured results). The present study demonstrated that the singlet excited state lifetime is associated with the detergents n-dodecyl-β-D-maltoside (DDM) and n-octyl-β-D-glucopyranoside (β-OG), but has nothing to do with the different sources of Cyt b 6 f complexes. Compared with the Cyt b 6 f dissolved in β-OG, the Cyt b 6 f in DDM had a lower fluorescence yield, a lower photodegradation rate of Chl a, and a shorter lifetime of Chl a excited state. In short, the singlet excited state lifetime, ～200 ps, of the Chl a in Cyt b 6 f complex in DDM is closer to the true in vivo. Supported by the National Natural Science Foundation of China (Grant Nos. 39890390 and 20673144)
%K cytochrome b
%K 6
%K f
%K detergent
%K chlorophyll a
%K singlet excited state lifetime
%K singlet oxygen<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=932756ED7E9856812AD6CE3AB23890E8&yid=A732AF04DDA03BB3&vid=771152D1ADC1C0EB&iid=E158A972A605785F&sid=42D7028D961473F8&eid=25467A5A28500A25&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=20