%0 Journal Article
%T cDNA cloning and expression of an apoptosis-related gene, human TFAR15 gene
%A WANG Yugang
%A LlU Uongtao
%A ZH ANG Yingmei
%A MA Dalong
%A <br>王玉刚
%A 刘洪涛
%A 张颖妹
%A 马大龙
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X By means of eDNA-RDA)A method, some cd)YA fragment- were found to have high levels oi expression during deprivation of GM-CSF ( granulocyte macrophage-rolony stimulating (actor) in a human myeloid cell line, TF-I cells. One of these fragments was identified as a novel gene. To gel the full length of eDNA, rapid amplification of cDYA ends (RACE) and expressed sequence tags ( EST) overlapting fragments assembling strategies were used. The novel gene was named TRAF15 (TF-1 cell apoptosis related gene-15) , which consist of I 218 nucleotides and eneodes 212 ammo acids. The putative protein product oi TFAR15 is parlially homologous to C. elegans protein C14A4. 11 . TFAR15 mRNA is expressed in fetal liver, kidney, spleen and lung. and also in some human myeloid cell lines. Both of the TFAR15 mRNA and protein in wen: highly expressed in TF-1 cells after GM-CSF withdrawal. In vitro analysis showed that the tecombinant TFAR15 protem coold inhibit the natural cell death of 293 cells, an embryonie kidney cell line.
%K apoptosis
%K cDNA-RDA
%K RACE
%K TF-1 cell line
%K 293 cells<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=AEC6FA3DD0B1A56F22EB4CAF9B6C6ADD&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=38B194292C032A66&sid=892C6E385D640C1E&eid=C6EC7357BCACD3A4&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=6&reference_num=0