%0 Journal Article
%T Coil-1 of rod domain of NF-L is essential for its assembly in vivo<br>
%A TONG Xiangjun
%A ZHAI Zhonghe
%A CHEN Jianguo
%A <br>
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X Neurofilaments take highly ordered structures composed of parallel mays of 10 nm filaments linked to each other with frequent cross-bridge. It is composed of three components named NF-L, NF-M and NF-H. NF-L is able to form filamentous network alone in Sf9 cells, while M could not. To identify which domain is essential for the assembly of NF-L, two chimera proteins named ML and MML were constructed: ML was composed of the head domain of NF-M and other domains of NF-L; MML was composed of the head and Coil-1 domains of NF-M and Coil-2 and tail domains of NF-L. ML was not only able to form filaments in Sf9 cells, but also co-assemble with NF-M into parallel filamentous bundles. MML could not assemble into filaments. Thus the Coil-1 domain of NF-L was essential for its assembly. Project supported by the National Grant for Outstanding Young Scientists.
%K neurofilament
%K assembly
%K hybrid protein<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=22E0DCB9275BFB079D397DCC2C7CC09C&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=94C357A881DFC066&sid=78BF76CF5B7CB0F2&eid=522844664D9E629A&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=11