%0 Journal Article
%T The Extraction and Primary Identification of Anthocyanidin in Euphorbia pulcherrima
一品红苞片花色素的分离及初步鉴定
%A Changquan Wang
%A Xiaobing Wei
%A
王长泉
%A 魏小兵
%J 植物学报
%D 2006
%I
%X Anthocyanidins in Euphorbia pulcherrima were extracted and identified by use of UV-vis spectrophotometry, high performance liquid chromatography (HPLC) and mass spectrometry (MS). Anthocyanidin extract in methanol showed 3 absorption peaks, at 270, 340 and 520 nm, in the UV-vis spectrum, with bathochromic and hypsochromic shift with methanol+AlCl3 and methanol+AlCl3+HCl, respectively; the ratio of the absorbance at 440 nm to that at 520 nm was 0.29. No fluorescence occurred under UV irradiance, and 5 absorption peaks occurred at 270 nm after HPLC. Five molecular ion peaks (m/z) occurred on MS: 595, 381, 611, 587 and 571, respectively, and monosaccharides in the extract were identified as rhamuose and glucose on HPLC. These results suggest that anthocyanidin in E. pulcherrima mainly include cyanidin 3-rutinoside, delphinidin 3-rutinoside, cyanidin 3- benzoylglucoside, delphinidin 3-benzoylglucoside and an unknown component.
%K
一品红
%K 花色素
%K 紫外-可见光分光光度计
%K 液相色谱
%K 质谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=27EC185000EEF1F696756370D6D3ACC5&yid=37904DC365DD7266&vid=EA389574707BDED3&iid=E158A972A605785F&sid=35E8A259891FB32F&eid=FA3423FC1AE95C4E&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=22