%0 Journal Article
%T Using yeast two-hybrid system to detect interactions of ATP synthase subunits from Spinacia oleracea<br>
%A SHI Xiaobing
%A WEI Jiamian
%A SHEN Yungang
%A <br>
%J 中国科学C辑(英文版)
%D 2000
%I Springer
%X Subunit interactions among the chloroplast ATP synthase subunits were studied using the yeast two-hybrid system. Various pairwise combinations of genes encoding α, β, γ, δ and ε subunits ofSpinach ATP synthase fused to the binding domain or activation domain of GAL4 DNA were introduced into yeast and then expression of a reporter gene encoding β-galactosidase was detected. Of all the combinations, that of γ and ε subunit genes showed the highest level of reporter gene expression, while those of α and β, a and ε, β and ε and β and δ induced stable and significant reporter gene expression. The combination of δ and ε as well as that of δ and γ induced weak and unstable reporter gene expression. However, combinations of α and γ, β and γ and α and δ did not induce reporter gene expression. These results suggested that specific and strong interactions between γ and ε, α and β, α and ε, β and ε and β and δ subunits, and weak and transient interactions between δ and ε and δ and γ subunits occurred in the yeast cell in the two-hybrid system. These results give a new look into the structural change of ATP synthase during catalysis.
%K ATP synthase
%K subunit interaction
%K yeast two-hybrid system<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=6736D6B231B49907A8B56E9A2A6CDF18&yid=9806D0D4EAA9BED3&vid=BE33CC7147FEFCA4&iid=0B39A22176CE99FB&sid=954CE65414DD94CA&eid=A58CF3BAE79427D0&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=17