%0 Journal Article
%T Dynamic changes of [Ca2+] i in cerebellar granule cells<br>Dynamic changes of [Ca2+]i in cerebellar granule cells exposed to pulsed electric fields
%A CHEN Ya
%A WANG Yan
%A SUN Tong ZHANG Jinzhu
%A JING Xianghong
%A LI Ruiwu
%A <br>
%J 中国科学C辑(英文版)
%D 2000
%I Springer
%X Intracellular free Ca2+ concentration (Ca2+]i) in embryonic chick cerebellar granule cells loaded with fluo-3/AM and exposed to a single pulsed electric field was investigated using a confocal laser scanning microscope and fluorescent microscope equipped with CCD video imaging system. The results showed that Ca2+]i increased immediately and rose to the peak rapidly as the cells exposed to a single pulsed electric field. The amplitude and rate of the increases of Ca2+]i depend on the intensity of external electric field. In the presence of Ca2+ chelant EGTA or Ca2+ channels blocker La3+ in the pulsation solutions, the increase of Ca2+]i was still observable. It was also observed that Ca2+]i of different intracellular areas in the cell elevated simultaneously while the peak of the increase of Ca2+]i in the poles of the cell preceded to the peak in its somata and recovered to a plateau within a short time.
%K pulsed electric field
%K Ca2+
%K granule cells
%K fluo-3/AM
%K confocal laser scanning microscopy<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=724CE7B1AFE9F412&yid=9806D0D4EAA9BED3&vid=BE33CC7147FEFCA4&iid=CA4FD0336C81A37A&sid=80A07035DF96B0C4&eid=35FC3610259C2B32&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=15