%0 Journal Article
%T The effect of cdk-5 overexpression on tau phosphorylation and spatial memory of rat
%A LIAO Xiaomei
%A ZHANG Yingchun
%A WANG Yipeng
%A WANG Jianzhi
%A <br>LIAO Xiaomei
%A ZHANG Yingchun
%A WANG Yipeng & WANG Jianzhi
%J 中国科学C辑(英文版)
%D 2004
%I Springer
%X In Alzheimer's disease (AD), hyperphosphorylation of tau may be the underlying mechanism for the cytoskeletal abnormalities and neuronal death. It was reported that cyclin-dependent kinase5 (cdk-5) could phosphorylate tau at most AD-related epitopes in vitro. In this study, we investigated the effect of cdk-5 overexpression on tau phosphorylation and spatial memory in rat. We demonstrated that 24 h after transfection into rat hippocampus, cdk-5 was overexpressed and induced a reduced staining with antibody tau-1 and an enhanced staining with antibodies 12e8 and PHF-1, suggesting hyperphosphorylation of tau at Ser199/202, Ser262/356 and Ser396/404 sites. Additionally, the cdk-5 transfected rats showed long latency to find the hidden platform in Morris water maze compared to the control rat. 48 h after transfection, the level of cdk-5 was decreased significantly, and the latency of rats to find the hidden platform was prolonged. It implies that in vivo overexpression of cdk-5 leads to impairment of spatial memory in rat and tau hyperphosphorylation may be the underlying mechanism.
%K cyclin-dependent
%K kinase5
%K (cdk-5)
%K tau
%K spatial
%K memory
%K Alzheimer抯
%K disease.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=55AD29C72EBF5B74CC2E472C9AFE5537&yid=D0E58B75BFD8E51C&vid=F4B561950EE1D31A&iid=38B194292C032A66&sid=4B1FFFA116F7AE3B&eid=4290346F7268639E&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=20