%0 Journal Article
%T Application of Cryo_sectioning Technique in Highly Lignified Tissues<br>高度木质化材料的冰冻切片技术
%A LIN Yue-Hui
%A LI Han-Bing
%A HE Xin-Qiang
%A <br>林月惠
%A 李寒冰
%A 贺新强
%J 植物学报
%D 2001
%I 
%X It is normally difficult to cut nice sections and prepare good micrographes from the highly_lignified tissues by means of conventional sectioning methods. In this paper, the cryo-sectioning was introduced to overcome the difficulty and it was further proved to be a fast, simple and effective way of section preparation without passing a long process of softening, dehydration and embedding. The crucial steps are to select a proper concentration of glycerin, a suitable temperature of freezing and an appropriate way of section_flatting.
%K <br>木质化材料
%K 冰冻切片
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=DC73B37ADD6CF18B2487D07F6FB6B037&yid=14E7EF987E4155E6&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=7F5DDA4924737DF5&eid=2B5DE8A23DCEED39&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=3