%0 Journal Article
%T Structural analysis of desheptapeptide(B24-B30) insulin by molecular replacement<br>
%A BAO Sujin
%A XIE Dianlin
%A ZHANG Jiping
%A CHANG Wenrui
%A LIANG Dongcai
%A <br>
%J 中国科学C辑(英文版)
%D 1998
%I Springer
%X Desheptapeptide (B24 B30) insulin (DHPI), a virtually inactive insulin analog, has been crystallized in space group P2\-12\-12\-1 with two DHPI molecules in an asymmetric unit. The orientations and positions of the molecules were determined by molecular replacement, and a structural model was built at 0.3 nm resolution. The current model shows that the two DHPI monomers are related by a non crystallographic 2 fold axis, nearly parallel to the crystallographic c axis. This structural feature complicated the determination of the orientation of the local 2 fold axis, which was later confirmed by analysing the diffraction data of DHPI crystals.
%K insulin
%K desheptapeptide (B24
%K B30) insulin
%K molecular replacement<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=2B0CCD71D55520916BCA65784BEEFE83&yid=8CAA3A429E3EA654&vid=2001E0D53B7B80EC&iid=38B194292C032A66&sid=B799C1769FCACDC8&eid=7ABC4505E3960D2B&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=13