%0 Journal Article
%T Microdissection of additional chromosome in common wheat-Th. intermedium TAI-27 and screening of its special probe<br>Microdissection of additional chromosome in common wheat-Th.intermedium TAI-27 and screening of its special probe
%A TIAN Chai
%A LU Yifan
%A DENG Jixian
%A LI Bin
%A ZHANG Xueyong
%A LIU Guangtian
%A <br>靫
%J 中国科学C辑(英文版)
%D 2000
%I Springer
%X It was identified that there were 4 St chromosomes derived from Th, intermedium in common wheat-Th. intermedium alien additional line TAI-27 by in situ hybridization. Two St chromosomes added to wheat chromosome in TAI-27 as well as two of them replaced two of 42 in wheat chromosomes. This indicates that TAI-27 is not only an alien additional line, but also a replacing line. The additional chromosomes in TAI-27 were microdissected and a microcloning library was constructed. A special probe of Th. intermedium was obtained from a microcloning library. The sequence analysis indicated that there were no homology with Genebank data. This makes it possible to screen genes with the disease-resistance, adversity-tolerance and fine character from Th.intermedium.
%K Th
%K intermedium
%K chromosome
%K microdissection
%K special probe<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=A60D96936FF42A14&yid=9806D0D4EAA9BED3&vid=BE33CC7147FEFCA4&iid=CA4FD0336C81A37A&sid=03F1579EF92A5A32&eid=4BB057F167CF3A60&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=3&reference_num=11