%0 Journal Article
%T Establishment and application of minigene models for studying pre-mRNA alternative splicing
%A LI Jing
%A CHEN Xianhua
%A LIN Wanmin
%A LI Lishu
%A HAN Yu
%A XU Ping
%A <br>LI Jing
%A CHEN Xianhua
%A LIN Wanmin
%A LI Lishu
%A HAN Yu & XU Ping Laboratory of Genomic Physiology
%A School of Life Sciences
%A Fudan University
%A Shanghai
%A China
%J 中国科学C辑(英文版)
%D 2004
%I Springer
%X The objective of the present study is to establish a minigene model for studying pre-mRNA alternative splicing. To prepare the minigene DNA constructs, with human or mouse genomic DNA as templates, GluR-B, FGF-2R and Zis "minigene" fragments were amplified using PCR and cloned to the eukaryotic expression vectors. The three constructed minigenes and the expression vectors of Tra2beta1 and Zis2 were co-transfected in Hela cells. RT-PCR analysis was performed to semi-quantitatively determine the spliced products from the minigenes. The results demonstrated that the constructed minigenes are useful in studying the pre-mRNA alternative splicing in cultured cells. With the established Zis minigene, we for the first time found that Zis2 isoform regulates the alternative splicing of Zis minigene.
%K minigene
%K GluR-B
%K FGF-2R
%K Zis
%K Tra2b
%K pre-mRNA splicing<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=9CD968C6B54224D06B9F91B5A7738B3D&yid=D0E58B75BFD8E51C&vid=F4B561950EE1D31A&iid=38B194292C032A66&sid=CC0ECB9C52F1B85F&eid=AD16A18DBD734D13&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=31