%0 Journal Article
%T An Improved Clearing and De-waxing Method for Plant Paraffin Sectioning<br>植物石蜡制片中透明和脱蜡技术的改良
%A Hubiao Yang
%A Xiaoxia Li
%A Lijuan Luo
%A <br>杨虎彪
%A 李晓霞
%A 罗丽娟
%J 植物学报
%D 2009
%I 
%X Paraffin sectioning plays an important role in plant science research. It is used widely in histology to observe and explain microstructure and provides a credible method for development of plant histology. This study aimed to investigate the limitations in traditional paraffin sectioning, such as the complex and time-consuming traditional protocol for improved measure. Xylene, a kind of neurotoxicant, is often used as a dewaxing and clearing chemical in the materials-preparation process. We substituted histolene (C1OH16), van-clear, oil of turpentine and n-butanol for xylene, to avoid a toxic effect. We improved the method to simplify the process of paraffin sectioning, shorten the cycle of the experiment and obtain quality slides.
%K <br>改良方案
%K 石蜡切片
%K 植物组织学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=A50AFCE1DE0C00F9B2809EC79AC760F2&yid=DE12191FBD62783C&vid=1AE5323881A5ECDC&iid=0B39A22176CE99FB&sid=D6354F61445E9456&eid=6CCE24D86D03D083&journal_id=1674-3466&journal_name=植物学报&referenced_num=5&reference_num=13