%0 Journal Article
%T Effects of clinorotation on COL1A1- EGFP gene expression
%A DAI Zhongquan
%A LI Yinghui
%A DING Bai
%A ZHANG Yuguo
%A LIU Weiquan
%A LIU Pengpeng
%A <br>DAI Zhongquan
%A LI Yinghui
%A DING Bai
%A ZHANG Yuguo
%A LIU Weiquan & LIU Pengpeng . Laboratory of Space Cellular and Molecular Biology
%A Institute of Space Medico-Engineering
%A Beijing
%A China
%A . College of Biological Science
%A Chinese Agriculture University
%A Beijing
%A China
%J 中国科学C辑(英文版)
%D 2004
%I Springer
%X Bone-formation related gene plays a critical role in bone loss induced by space mi-crogravity, however the exact mechanism is unclear. In this study, we aim to investigate the effect of microgravity on the activity of ?1(I) collagen (COL1A1) gene promoter and the expression of osteoblast-related genes. COL1A1 promoter was digested by restriction enzymes resulting in three DNA fragments. The fragments were ligated with the enhanced green fluorescent protein report gene, and subcloned into expression vectors. ROS17/2.8 cells transfected by these vec-tors were screened by G418, and enhanced green fluorescent protein (EGFP) positive colonies were isolated and cultured under clinostat condition. EGFP and Collagen type I expression level were detected by fluorescence intensity analysis and immunocytochemistry methods respectively. The results showed that the expression of EGFP and collagen type I was increased 24 h, 48 h after the cells were cultured under stimulated microgravity, illustrating that the activity of COL1A1 promoter might be increased. In conclusion, osteoblasts can compensatively increase the ex-pression of type I collagen by enhancing the activity of COL1A1 promoter under short-term simulated microgravity conditions.
%K simulated microgravity
%K osteoblast
%K gene expression
%K COL1A1 promoter<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=16EA5A43066622E6AFC9AA4D5D7DC9F8&yid=D0E58B75BFD8E51C&vid=F4B561950EE1D31A&iid=38B194292C032A66&sid=38685BC770C663F2&eid=AA27B676BFCAA4BE&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=6&reference_num=20