%0 Journal Article
%T Embryo Germination and Rapid Propagation of Rare and Endangered Plant Davidia involucrata
珍稀濒危植物珙桐胚的萌发与快速繁殖
%A 余阿梅
%A 苏智先
%A 王立强
%A 方志荣
%A 邹利娟
%J 植物学报
%D 2009
%I
%X Three factors (media type, gibberellic acid GA3] and active carbon) affecting the embryo germination of Davidia involucrata were optimized by use of a uniform design and analyzed by regression analysis . Then a system for micropropagating D. involucrata was established from the embryos . From regression equations, the optimal conditions for embryo germination were the addition of 2.85 mg.L-1 GA3 and 0.25 g.L-1 activated carbon to 1/2 MS medium containing 0.5 mg.L-1 IBA and 1 mg.L-1 6-BA. The germination rate of embryo was 87.72%, without significant deviation to the predicted value. The optimal conditions for shoot induction and multiplication were WPM medium supplemented with 0.1 mg.L-1 NAA and 2.0 mg.L-1 6-BA; the mean shoots obtained per explant was 4.34. An amount of 90% of shoots rooted on rooting medium, and 71% plants survived after transplanting.
%K
珙桐
%K 胚
%K 回归分析
%K 均匀设计
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=41BC271D929F709AC14EB9EC3A386241&yid=DE12191FBD62783C&vid=1AE5323881A5ECDC&iid=E158A972A605785F&sid=ABF2590617D31FFD&eid=C4490A71BEB872FA&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=22