%0 Journal Article
%T Sensitivity of Plant Leaves at Different Developmental Stages to UV-induced DNA Damage<br>紫外辐射诱导植物叶片DNA损伤敏感性差异
%A Jing Wang
%A Lei Jiang
%A Yan Wang
%A Shaoshan Li
%A <br>王静
%A 蒋磊
%A 王艳
%A 李韶山
%J 植物学报
%D 2007
%I 
%X Comet assay or single-cell gel electrophoresis is now widely used to detect DNA damage in cultured animal cells, but only a few reports describe the application of comet assay in plant cells. Here, the accepted animal cell protocol was modified to adapt it to plant cells and the sensitivity to UV-B induced DNA damage was investigated in young and mature leaves of Murraya panicuata. Both UV-A and UV-B irradiation induced DNA damage in mesophylls. The level of DNA damage was much higher in younger than mature leaves, which indicates that young leaves are more sensitive to UV-B radiation than mature leaves.
%K <br>彗星检测
%K DNA损伤
%K 单细胞凝胶电泳
%K UV-B辐射
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=0AA08AA8188AE313669B31C780CEFA86&yid=A732AF04DDA03BB3&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=3A0155B37D8FF829&eid=23104246A5FCFCEF&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=22