%0 Journal Article
%T Asialoglycoprotein receptor and liposome synergistically mediate the gene transfer into primary rat hepatocytes
%A LI Chonghui
%A <br>李崇辉
%A 温守明
%A 翟海峰
%A 孙曼霁
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X Gene transfer into primary rat hepatocytes was performed by employing cationic liposome as DNA carrier and the specific ligand of hepatic asialoglycoprotein receptor (ASGPR), asialofetuin, as liver-targeting ligand. The results showed that asialofetuin, when added to the gene transfer complexes, could significantly increase the hepatocyte transfection efficiency, and alleviate the cellular toxicity of Lipofectin. Several synthetic ligands of ASGPR (galactosyl albumin) could also increase the transfection efficiency of hepatocyte like asialofetuin. It was proved that ASGPR and cationic liposome could synergistically mediate the gene transfer into primary rat hepatocytes. This novel gene delivery system provided a safer, more simple and efficient gene transfer method for primary hepatocytes, and showed prospecting application in hepatic gene therapy.
%K asialoglycoprotein receptor
%K cationic liposome
%K receptor-mediated
%K gene transfer
%K primary hepatocyte<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=250DC51C9EAE3858BCD14B1050A81DC6&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=E158A972A605785F&sid=8D75AD3BD0D1BCC5&eid=78976D931AD1540F&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=13