%0 Journal Article
%T Expression of recombinant rat Neurotrophin-3 in Chinese hamster ovary cells
%A JI AiminSHU SiyunLI Ming BAO XinminZOU Hengqin
%A ZHANG Zhongyi
%A <br>季爱民
%A 舒斯云
%A 包新民
%A 邹恒琴
%A 张忠义
%A 李明
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X The CHO cell line stably producing recombinant rat NT-3 was established. The insertion of rNT-3 cDNA into transferred cell gonome was analyzed with Southern blot. The expressed protein was identified by Dot ELISA (enzyme-linked immunosorbent assay) and Western blot. Western blot showed a clear specific band of about 14 ku for NT-3. The mean level of rNT-3 in four NT-3cDNA/CHO cell lines was about 2 100 ng/106 cells/48 h determined by EIA. The conditioned-medium (CM) of NT-3cDNA/CHO cells could promote the fiber outgrowth of the dissociated dorsal root ganglion of 8-day-old chick embryos, which shows a dose-response relationship. A half-maximal concentration of the biological activity (EC50) of the recombinant protein was approximately 16.7 ng/mL. The MoAb 3W3 of NT-3 could neutralize the biological activity of the rNT-3.
%K Neurotrophin-3
%K cDNA
%K recombinant
%K nerve fiber outgrowth<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=28DFEA4F2E532BDCD67330954BD98DB6&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=B31275AF3241DB2D&sid=04FC77FB58A9B53A&eid=A0C7970CD30381EA&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=14