%0 Journal Article
%T Preliminary Study of the Induction of Transformed Hairy Roots and Solenesol Content in Nicotiana tabacum
烟草毛状根诱导及其茄尼醇含量初探
%A Yingjuan Wang
%A Huaiyu Bu
%A Duowei Li
%A Jingfeng Jia
%A
王英娟
%A 步怀宇
%A 李多伟
%A 贾敬芬
%J 植物学报
%D 2006
%I
%X Solanesol is an important material for synthesizing ubiquinone. An efficient transformation system was established in Nicotiana tabacum by use of Agrobacterium rhizogenes W.T15834.The content of solanesol was detected. Hairy roots could grow normally on solid or liquid MS medium without a growth regulator. However, the hairy roots grew rapidly and no callus formed during subculture in liquid medium. The transformation of hairy roots was confirmed by PCR and mannopine assay. By use of improved HPLC, we obtained a content of solanesol of almost 43.2% in hairy roots that was 1.12 times that in roots of tobacco seedlings, but still lower than that in discarded tobacco leaves; the content of solanesol is normally higher in liquid culture than in solid culture. This study aims to provide a basis for future research in taking advantage of hairy root technology to produce solanesol.
%K
烟草
%K 发根农杆菌
%K 毛状根
%K 茄尼醇
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=B1BCDB3F4210EFB067F69DD3D823DEC5&yid=37904DC365DD7266&vid=EA389574707BDED3&iid=E158A972A605785F&sid=9DC563A0FEFC04F9&eid=9D9F10A828991FA6&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=16