%0 Journal Article
%T Temperature dependence of intracellular free calcium in cardiac myocytes from rat and ground squirrel measured by confocal microscopy
%A WANG Shiqiang ZHOU ZengquanQIAN Hong
%A <br>王世强
%A 周曾铨
%A 钱洪
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X The temperature-dependence of infraeeliular free caleimn (Ca) was investigated in mdo-1 loaded ventricular myocytes from the ral, a non-hibernator, and from the ground squirrel, a hibernator. The dissociation constant of indo-l at different temperatures was calibrated both al pll-tat and at @-stat . and the result demonstrated that the @-stat ralibration should be prettrred . Analysis of the fluoreseent image showed a striking increase of Ca2 as well as spontaneous caleiuni waves in ral cells, indicating an overloaded cakuum. In contrast, cardiac myocytes of the ground sqnirraf were found to keep a constant (Ca2 ) without caleium overload regardless of temperature variation. It is be-lieved that understanding of the mechanisms underlying the interccllular caleima homeostasis of hibrernators may lead to solutions of some medical questions .
%K cardiac myocyte
%K calcium homeostasis
%K hibernation
%K temperature
%K confocal laser-scanning microscopy
%K fluorescent calcium probe<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=09A57BA12E84555BD0FC1FEFBE690EE0&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=38B194292C032A66&sid=2AC7DCCBBC26ECF8&eid=BF112261B65CB9C9&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=0