%0 Journal Article
%T Molecular cloning, sequence analysis and expression studies of a novel GAmyb homologous gene, hvmyb<br>
%A LING Shiyun
%A QI Fang
%A ZHU Yuxian
%A <br>
%J 中国科学C辑(英文版)
%D 1998
%I Springer
%X Using a knownGAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homoIogous to the 3′ termini ofGAmyb (97%) and their 3′ termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putativeGAmyb activation domain but lack the normal DNA-binding domain. Northern blot reveals thathumyb expression in barley aleurone layers is strongly up-regulated by gibberellin (GA) and down-regulated by abscisic acid (APIA). The tissue-and developmental-stage-specificity ofhvmyb was also found, which was only expressed in barley aleurone cells and dropped to non-detectable level soon after germination. EMBL accession number Y14658.
%K barley
%K aleurone
%K GAmyb
%K GA
%K ABA<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=00AD8877D7AA3E258DE202AE765E3746&yid=8CAA3A429E3EA654&vid=2001E0D53B7B80EC&iid=94C357A881DFC066&sid=20C3B205768D55E0&eid=2A2AA8B7E19F0DF7&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=15