%0 Journal Article
%T Comparison of Five Methods of DNA Extraction From Pinus massoniana<br>五种提取马尾松基因组DNA方法的比较
%A LI Dan LING Ding-Hou
%A <br>李丹
%A 凌定厚
%J 植物学报
%D 2000
%I 
%X Conifers are high in polysaccharides and other secondary metabolites such as polyphenols, terpens and resins, which makes it rather difficult to obtain high quality genomic DNA from their tissues. Five methods,i.e. basic method, high salt precipitation, CTAB precipitation, Ziegenhagen's method and DNeasy Plant Mini Kits(QIAGEN), were used to extract genomic DNA from the needle leaves of WTBX]Pinus massoniana.WT] The resulted DNA samples of the five methods were tested using agarose gel electrophoresis, restriction digests and PCR. The difference in yield, quality, time duration and expense of the obtained DNA are compared, and CTAB precipitation was decided as the best methed for the practical work of molecular ecology.
%K <br>二次代谢产物
%K 基因组DNA
%K 马尾松
%K 分子生态学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=A3C274C9A99225778B3E14BCFCC92DA3&yid=9806D0D4EAA9BED3&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=BBF7D98F9BEDEC74&eid=DABEF202280E7EF1&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=0