%0 Journal Article
%T Genetic mapping of a new semi-dwarf gene, sd-t(t), in indica rice and estimating of the physical distance of the mapping region<br>Genetic mapping of a new semi-dwarf gene,sd-t(t),in indica rice and estimating of the physical distance of the mapping region
%A JIANG Guanghuai
%A LIANG Guohua ZHAI Wenxue GU Minghong LU Runlong XU Jichen ZHU Lihuang
%A <br>江光怀
%A 梁国华
%A 翟文学
%A 顾铭洪
%A 鲁润龙
%A 徐吉臣
%A 朱立煌
%J 中国科学C辑(英文版)
%D 2002
%I Springer
%X Application and functional study of dwarf and semi-dwarf genes are of great importance to both crop breeding and molecular biology. A new semi-dwarf gene, sd-t(t), non-allelic to sd-1, had been identified in an indica rice variety, Aitaiyin 2. In this study the gene was genetically mapped by using an F2 population, which consisted of 474 individuals developed from a cross between Aitaiyin 2 and B30. The sd-t(t) gene was located between the RFLP markers R514 and R1408B with a distance of 1.1 cM to R514, and 4.5 cM to R1408B on chromosome 4. A physical contig covering the sd-t(t) mapping region was further constructed by screening a BAC library with R514 and R1408B as probes, and the physical distance between R514 and R1408B was estimated at approximately 147 kb. This result will facilitate map-based cloning of the sd-t(t) gene.
%K semi-dwarf gene sd-t(t)
%K simple sequence length polymorphism (SSLP)
%K restriction fragment length polymorphism (RFLP)
%K molecular marker
%K genetic mapping
%K BAC contig<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=27499FC480E450282CCE68B91BC811E8&yid=C3ACC247184A22C1&vid=94E7F66E6C42FA23&iid=E158A972A605785F&sid=3BAAE0DA6093AC05&eid=7D6CD8918B045FD4&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=26