%0 Journal Article
%T Study of thrombopoietin for gene therapy of thrombocytopenia
%A CHONG Song
%A LU Daru
%A LI Changben
%A QIU Xinfang
%A XUE Jinglun
%A <br>崇松
%A 卢大儒
%A 李昌本
%A 邱信芳
%A 薛京伦
%J 中国科学C辑(英文版)
%D 1999
%I Springer
%X Thrombopoietin (TPO) is likely to be a potent, specific and reliable medication in the treatment of throm-bocytopenia. A TPO-highly-expressed plasmid pcDNA3-TPO was constructed and a primary study was made on the expression of TPO cDNA in vitro and gene transfer study for thrombocytopenia in vivo. rhTPO showed complete and stable bioactivity by a series of indicators. High expression of TPO was detected in plasma from healthy mice or thrombocytopenia mice model receiving direct intramuscular injection of pcDNA3-TPO. And the platelet level of healthy mice peaked to 1.9-fold of baseline. Mice with CTX- induced thrombocytopenia achieved profound nadirs, acceleration of recovery, even 1.8-2.0-fold supranormal levels of peripheral platelet counts. The results offered experimental support for clinical application of gene therapy for thrombocytopenia via direct intramuscular injection of TPO cDNA.
%K thrombopoietin (TPO)
%K gene transfer
%K thrombocytopenia
%K intramuscular injection<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=D9F9C670099CE089B451E212F6D1B0B3&yid=B914830F5B1D1078&vid=ECE8E54D6034F642&iid=B31275AF3241DB2D&sid=E543FC2C7CA75C74&eid=4720E9D07E8A2290&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=15