%0 Journal Article
%T ESI-MS study on non-covalent bond complex of rhFKBP12 and new neurogrowth promoter
%A GU Li
%A YAO Weijuan
%A YAN Zongyi
%A XIE Lide
%A SUN Dagong
%A LI Dan
%A ZENG Zhu
%A WEN Zongyao
%A <br>王红霞
%A 张学敏
%A 杨松成
%A 肖军海
%A 聂爱华
%A 赵丽琴
%A 李松
%J 中国科学C辑(英文版)
%D 2003
%I Springer
%X An ESI-MS method for studying the non-covalent bond complex of rhFKBP12 with its nonimmunosuppressive ligands was developed. The method was used to screen out three com-pounds capable of binding to rhFKBP12 non-covalently from 52 compounds. By competing binding experiment, the binding site and the relative binding strength of these three compounds 000107, 000308 and A2B12 with rhFKBP12 were measured. All of them have the same binding site as FK506 does. X-ray crystalline diffraction experiment of non-covalent bond complex of 000107, 000308 with rhFKBP12 by Tsinghua University showed the same results. Among them 000308 has good effect on stimulating neurite to grow in chicken sensory neuronal cultures.
%K rhFKBP12
%K nerve growth promoter
%K non-covalent bond complex
%K ESI-MS<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=41AC7549FD85F93FB9BE08F837807851&yid=D43C4A19B2EE3C0A&vid=D997634CFE9B6321&iid=38B194292C032A66&sid=11CEECA6DA9E4AC5&eid=8BB50A069C48D50B&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=1&reference_num=9