%0 Journal Article
%T Comparison and Optimization of the Methods on Protein Extraction and SDS-PAGE in Woody Plants<br>木本植物蛋白提取和SDS-PACE分析方法的比较和优化
%A GU Rui-Sheng
%A LIU Qun-Lu
%A CHEN Xue-Mei
%A JIANG Xiang-Ning
%A <br>谷瑞升
%A 刘群录
%A 陈雪梅
%A 蒋湘宁
%J 植物学报
%D 1999
%I 
%X The methods of protein extraction and SDS-PAGE were compared and optimized in woody plants. The improved steps included modified protein extraction, substitution of ethanol for methanol in the solution of staining and destaining. Satisfied results were achieved in the application of the optimized method to the protein analysis of several woody plants. 66 kD protein band related to salt stress and 28 kD protein band associated with osmotic stress were obviously showed out in the analysis for the suspensivly cultured cells stressed with NaCl of Populus euphratica.
%K <br>木本植物
%K 蛋白提取
%K 十二烷基磺酸钠聚丙烯酰胺凝胶电泳
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=7C3BBF84F668ECB3A8D8BBEA3FFC34DF&yid=B914830F5B1D1078&vid=7801E6FC5AE9020C&iid=0B39A22176CE99FB&sid=73579BC9CFB2D787&eid=6425DAE0271BB751&journal_id=1674-3466&journal_name=植物学报&referenced_num=2&reference_num=0