%0 Journal Article
%T Effect of recombinant growth hormone on expression of growth hormone receptor, insulin-like growth factor mRNA and serum level of leptin in growing pigs
%A XU Qingfu
%A ZHAO Zhihui
%A NI Yingdong
%A ZHAO Ruqian
%A CHEN Jie
%A <br>胥清富
%A 赵志辉
%A 倪迎冬
%A 赵茹茜
%A 陈杰
%J 中国科学C辑(英文版)
%D 2003
%I Springer
%X Sixteen Large White x Landrace castrated male pigs were allotted into treatment and control group. The treatment group was injected intramuscularly with recombinant porcine growth hormone (rpGH, 4 mg d(-1)) and the control group with vehicle for 28 days. Animals were slaughtered 4 h after final injection for liver, longissimus dorsi (LD) muscle and blood sampling. Serum concentration of insulin-like growth factor 1 (IGF-I) and leptin were determined by RIA. The total RNA was extracted from tissues to measure the abundance of growth hormone receptor (GHR), IGF-I mRNA by RT-PCR with 18S rRNA internal standard. Results showed that rpGH enhanced the average daily weight gain by 26.1% (P < 0.05), the serum IGF-I concentration by 70.94% (P < 0.01), decreased serum leptin by 34.8% (P < 0.01). The relative abundance of GHR and IGF-I mRNA in liver were increased by 24.45% (P < 0.05) and 45.30% (P < 0.01), respectively, but no difference of GHR (P > 0.05) and IGF-I mRNA (P > 0.05) in LD between GH treated and control group was found. These results suggest that rpGH can up-regulate hepatic GHR and IGF-I gene expression and improve animal growth. However the effect of rpGH on GHR and IGF-I gene expression are tissue-specific.
%K pigs
%K growth hormone
%K insulin-like growth factor ? growth hormone receptor<br>insulin-like
%K growth
%K factor
%K Ⅰ
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=180CF3A72E750F3261A8A60EDC957784&aid=8D03DEC46EBC026B9ABFB6214AB4AA38&yid=D43C4A19B2EE3C0A&vid=D997634CFE9B6321&iid=0B39A22176CE99FB&sid=EF27C460877D3C9F&eid=CC0ECB9C52F1B85F&journal_id=1674-7305&journal_name=ScienceChina.Lifesciences&referenced_num=0&reference_num=25